2020. 3. 6. 06:55ㆍ카테고리 없음
Imaris Open - Debate ForumThis will be a community discussion room where Imaris is definitely the key subject. This discussion board will offer assistance to fresh users, technical as nicely as application support. Customers from websites with a valid Imaris Infinity membership will get priority - however, the fastest method to get to the Bitplane Complex and Program Support group will keep on to be via Asia, EU or US. Every signed up user can add items to the community forum. Non-registered customers can go through the discussion board but cannot add more/comment, etc. All ideas, questions, suggestions and feedback are encouraged. The problems with launching the lined up tiles straight into Imaris relating to the present metadata in the CZI document about the counter is definitely that currently Imaris doesn'capital t support loading of tiles with overlapping sides.
So the XTension would possess to not only handle loading the tiles into the right roles, but furthermore how to process the intensities in the areas where the sides overlap.Definitely this is usually achievable, but can make the XTension concept considerably even more work to apply, and adds more computational fill. In reality, your demand is not really very various from the outdated 'stitch datasets' XTénsion, except that loading several tiles from the CZI structure has not really been attempted. That XTension was 'retired' due to overall performance issues, and the look on the scene of XUVstitch which had been considered a much better tool for the work - but, I speculate we should appear into whether the efficiency issues can end up being prevented if the Zeiss Zen software has currently taken care of the job of refining the tile positions. I don't recognition exactly what the bottleneck had been now.Apart from searching into reviving that old sewing XTension, at the instant, there are usually two present options for a workaround:Option A: In the Zeiss Zen software, under the Stitching section, choose New Output - Fuse Tiles. This will conserve a document with a individual, large, fused picture, that Imaris can download directly.Choice B: If you put on't have got access to Zen software program, make use of Imaris Document Converter to convert the CZI file into a series of IMS documents - the counter placement (phase/focus placement) should end up being maintained in the métadata of the lMS documents. Then, insert the IMS data files into XUVstitch.
You can neglect the Central processing unit and RAM heavy phase of computing the alignment, if you believe in the placement refinement was well dealt with in Zen, and simply make use of the existing roles, and conserve the task as a individual IMS document.Take note 1: XUVstitch does not compute multi-resolution information when preserving IMS data files by defauIt, but you cán switch in on in the options after you start the software.Take note2: XUVstitch utilizes BioFormats, but an outdated edition. If it had been up to date to the most recent BioFormats version that supports CZI data files, you could skip the step of converting to IMS files.Note3: XUVstitch needs multiple Z-planes for floor tile position. But if you put on't operate the position refinement functions, and just make use of the software program to re-save the task as a solitary image with the preliminary placements, it appears to also function for 2D tiles.
2/3(1+n)=-1/2n
Regrettably time-lapse pictures are not really supported.
Mathway
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